Lactophenol cotton blue used for wet mounts in the examination of yeasts and molds, serving as mounting fluid and stain. Jul 20, 2014 in this prospective study, which was carried out from march to september, 20, we have evaluated the performance of iodineglycerol in comparison to lactophenol cotton blue lpcb and congored formaldehyde as an alternative method for identification of filamentous fungi. Lactophenol cotton blue stain other means of identification. Staining of fungus by using lactophenol cotton blue. The yeast with lactophenol cotton blue is called a simple stain because it stains the yeast cells, so they can be more readily observed under the microscope.
The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse. Material safety data sheet section 1 product and company information product name. It is to be used only by adequately trained and qualified. Lactophenol cotton blue is a stain that is used to examine fungal elements following either a tape preparation or a scraping. Eng scientific lactophenol cotton blue stain, certified. Gram stain and lactophenol cotton blue stain are used to visualize certain fungal structures that are significant for identification.
Learn vocabulary, terms, and more with flashcards, games, and other study tools. Although many of the essential oils are terpenes, some are phenolic compounds, for example thymol from thymus spp. Uses advised against no specific uses advised against are identified. Jorvet j326b lactophenol cotton blue stain for animal use. Add one or two drops of lactophenol cotton blue stain before alcohol gets off. Principle lpcb is a stain used for making semipermanent microscopic preparation of fungi. Sterilize the needle and cool it then transfer a mycelial mat on fluid and press it gently so that it easily mix with the stain. Remove excess stain and mount in lactoglycerol or water. Phenols constitute probably the largest group of plant secondary metabolites, varying in size from a simple structure with an aromatic ring to complex ones such as lignins. Lactophenolcotton blue solution, readily available from biological supply houses, stain fungi blue only living tissue picks it up. Supplied in crushable ampules enclosed in a protective plastic sheath with a convenient dropper dispenser tip.
Cotton blue lactophenol page 1 of 8 material safety data sheet according to 91155ec section 1. Preparation of lactophenol cotton blue slide mounts. Lactophenol aniline blue may be adequate for presumptive identification, however, colonial morphology and further microscopic examination may be necessary for definitive identification. Lactophenol cotton blue lpcb for the staining and microscopic identification of fungi. Search results for lactophenol blue solution at sigma aldrich. Cotton blue is an acid dye that stains the chitin present. Bactidroptm lactophenol aniline blue safety data sheet date of issue 27092007. Sigmaaldrich 3050 spruce street saint louis mo 63103 usa telephone. A traditional stain which must be handled with care.
Lactophenol anilin blue solution, lactophenol cotton blue solution 635. The material is stained in one step with lactophenol blue solution. Lactophenol, staining solutions, by loba chemie, india. What safe alternatives are there to lactophenol cotton. Lactophenol cotton blue stain fungal element stain for. Examine under microscope using 10x and 40x objective. As it is harmful to living cells it is no longer used, but may be used for cleaning surfaces and equipment. A solution of phenol and lactic acid in glycerol and water. Stain fungal elements with thermo scientific remel lactophenol aniline blue. Remel bactidrop lactophenol aniline blue is for staining fungal elements. Lactophenol aniline blue is recommended by koneman, roberts, and wright for direct mounting and staining of fungi. Dropper contains oneday supply of reagent or stain, hermetically sealed inside an ampule. Lactic acid acts as a preservative and it preserves the structure of fungus. Lpcb100, lpcb500, lpcba100, lpcba500 recommended use of the chemical and restriction on use.
Mix the specimen whether a skin scraping, fluid exudate or tissue with two drops of the 10% koh on a clean slide. Lactophenol blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. Add a drop of lactophenol cotton blue solution on a slide. Product name lactophenol blue solution sigmaaldrich. The slide can be left overnight to dry and later sealed with nail polish. Search results for lactophenol cotton blue at sigmaaldrich. Lactophenol anilin blue solution, lactophenol cotton blue solution. Bbl lactophenol cotton blue stain droppers are used in wet amounts for examination of fungi 50sp, 500ca. The use of lactophenol cotton blue lpcb stain for wet mount preparation of stools to demonstrate intestinal parasites by routine microscopy was evaluated in. Details of the supplier of the safety data sheet supplier prolab diagnostics 3. Organisms suspended in the stain are killed due to the presence of phenol. Linder recommended lactophenol as a mounting fluid for examination and preservation of fungi. Bd diagnostic 261188 dropper lactophenol stain, cotton.
The lactophenol cotton blue wet mount is simple and widely used staining method for fungi. Use of safe work practices are recommended to avoid eye or skin contact and inhalation of vapours. Relevant identified uses of the substance or mixture and uses advised against identified uses laboratory reagent. State the scientific name of the yeast studied in the laboratory you will use yeast over and over in. This readytouse solution is especially for airdried, heatfixed smears of bacteriological material, e. Lactophenol cotton blue stain is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Pdf evaluation of lactophenol cotton blue for wet mount. Do not ingest, inhale, or allow to come into contact with skin. Methyl blue is a chemical compound with the molecular formula c 37 h 27 n 3 na 2 o 9 s 3. Dissolve 20 g carbolic acid solid, 20 ml lactic acid, and 40 ml glycerol into 20 ml distilled water heat as gently as possible. Page 47 safety data sheet according to whs regulations printing date 29. Immediately flush eyes with gentle but large stream of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Slide culture technique and lactophenol cotton blue. For pigmented strains of moulds, lactophenol without cotton blue is available and can be used quality control internal quality control of the lactophenol blue solution must be performed regularly on known reference organisms to ensure the performance of the mounting solution.
Slide culture technique and lactophenol cotton blue staining for. Lactophenol cotton blue s016 intended use lactophenol cotton blue is used for staining solution for fungi. Aug 09, 2017 here in this mounting method we use lactophenol cotton blue stain where lactophenol acts as a cleaning agent as phenol is fungicidal in nature so it kills the organism. Evaluation of lactophenol cotton blue for wet mount preparation of. Fungal structures an overview sciencedirect topics. The lactophenol cotton blue lpcb wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. Add a drop of mounting fluid that is lactophenol cotton blue solution on a slide. Lactophenol cotton blue lcb is a mixture of methyl blue, a histological stain, and lactophenol it is used in wetmount preparations for visualization of fungal. Comparison of microscopic morphology of fungi using.
Check for and remove contact lenses if present and easy to do. Central drug house p ltd 728 vardaan house new delhi2 india. Some article used methylen blue stain but i dont know about the concentration that i should use. The lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. Lpcb is a stain used for making semipermanent microscopic preparation of fungi. Revisions highlighted packing group classification and labelling have been performed according to eu. Lactophenol cotton blue solution material safety data. Nested pcr is a modification of pcr designed to increase the sensitivity and specificity of the assay reaction. Place a drop of seventy percent alcohol on a clean microscope slide. It can be used in some differential staining techniques such as mallorys connective tissue stain and gomori trichrome stain, and can be used to mediate electron transfer in microbial fuel cells. What safe alternatives are there to lactophenol cotton blue. Composition ingredients formula adjusted, standardized to suit performance parameters directions principle and interpretation fungus are eukaryotic organism and they are classified into two main groups that is yeast and molds. A safer alternative to lactophenol for mycology is also available cotton blue in lactic acid.
Proslide microscope slides, frosted, premium, 3x1 inches x 1mm, 72 per box, by hardy diagnostics. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of. Invert the dropper for convenient dropbydrop dispensing. Place suspected fungi on a glass slide, drop lactophenolcotton blue solution on it, cover it with a cover slip and examine with an optical microscope. Identification of the substancemixture and of the companyundertaking 1. Pdf the use of lactophenol cotton blue lpcb stain for wet mount.
Molds examined in teased mounts must be well teased in order. Safety data sheet bactidroptm lactophenol aniline blue. Product name lactophenol cotton blue product number pl. Bd diagnostic 261188 dropper lactophenol stain, cotton blue. Place the coverslip on the drop avoiding air bubbles to be trapped.
Filter the cotton blue and distilled water solution into the phenolglycerollactic acid solution. Many simple phenols are responsible for taste, for example eugenol in. Jan 11, 2020 the lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. Lactophenol blue solution stain fungal, sigmaaldrich vwr. State the scientific name of the yeast studied in the laboratory you will use yeast over and over in this lab so know this cold for exams and quizzes. Lactophenol blue solution is readytouse staining solution for fungi staining in bacteriology with samples of human origin. Lactophenol cotton blue, fungal stain, 15ml, by hardy diagnostics. Z7 the hardy diagnostics manufacturing facility and quality management system comply with the fdas good manufacturing practices gmp and have achieved iso 485 certification as a medical device. Hermetically sealed in ampule oneday supply dropbydrop dispensing directions to perform tests, squeeze the dropper to crush the ampule. Bluemount, permanent fungal stain, lactophenol cotton blue with pva, 15ml each, by hardy diagnostics, prefer to ship ground. Slide culture technique and lactophenol cotton blue staining. Jorvet j326b lactophenol cotton blue stain indications.
Other information msds first issued 28022007 msds data revised 27092007 uses stain for fungal elements. Lactophenol cotton blue stain definition of lactophenol. Consult appropriate references for further instructions. It involves the use of two primer sets directed against the same target and two successive pcr. In this prospective study, which was carried out from march to september, 20, we have evaluated the performance of iodineglycerol in comparison to lactophenol cotton blue lpcb and congored formaldehyde as an alternative method. This item is sold and shipped to schools and businesses only. I read some of journal and they used lactophenol cotton blue stain but its hard to find it here. Page 17 safety data sheet according to whs regulations printing date 29.
This stain contains phenol, which will kill the organisms, lactic acid which preserves fungal structures, and cotton blue which stains the chitin found in the fungal cell walls. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. To perform tests, squeeze the dropper to crush the ampule. It is used as a stain in histology, and stains collagen blue in tissue sections.
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